HiTrap IXSelect: pour la purification du facteur de coagulation IX. HiTrap IgY: designed for purification of IgY. HiTrap IgM: designed for purification of monoclonal IgM. HiTrap MabSelect™, MabSelect SuRe™ and MabSelect Xtra™: developed to meet the productivity and sanitisation requirements of industry to develop large-scale antibody purification procedures. HiTrap Protein A and HiTrap Protein G: designed for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants. HiTrap NHS: high-performance medium designed for easy coupling of ligands containing primary amines. HiTrap Capto™: designed to meet industrial requirements for the production of biological molecules. HiTrap Capto Q chromatography column is packed with a strong anion exchange modern resin for high resolution, small-scale protein purification. It is possible to connect columns to each other for achieving columns of variable volume from 1 to 20 ml as requiredĪcronyms and abbreviations: Q = Quaternary ammonium / DEAE = Diethylaminoethyle / CM = Carboxymethyle / S = Methylsulfonate / ANX = Diethylaminopropyle Capto Q has a strong quaternary ammonium (Q) anion exchanger coupled to a chemically modified, high-flow agarose matrix. Each HiTrap™ column is equiped with one 1/16" male luer and one 1/16" female luer. HiTrap Capto Q is a strong anion exchanger prepacked with BioProcess Capto media for screening, and small-scale protein purifications using ion exchange chromatography (IEX).Both remain charged and maintain high capacity over broad pH ranges. Table 1.The active groups of Capto S, Capto Q and Capto DEAE. Media properties SP Sepharose High Performance and Q Sepharose High Performance are strong cation and strong anion exchangers respectively. HiTrap columns are available in 1 ml and 5 ml bed volume 2 Properties of Capto S, Capto Q and Capto DEAE For ion exchange chromatography, Capto S uses a sulfonate group, Capto Q uses a quartenary amine group and Capto DEAE uses a diethylaminoethyl group, as shown in the table below.HiTrap IXSelect: pour la purification du facteur de coagulation IX. Capto S, Capto Q and Capto DEAE are, respectively, strong cation, strong anion and weak anion exchange media for packed bed chromatography that increase. HiTrap IgM: designed for purification of monoclonal IgM. Capto Q, Capto S and Capto DEAE are ion exchange BioProcess media for capture and intermediate purification of proteins.
HiTrap NHS: high-performance medium designed for easy coupling of ligands containing primary amines. HiTrap Capto Q ImpRes chromatography column is packed with a strong anion exchange modern resin for high resolution, small-scale protein purification.
It is possible to connect columns to each other for achieving columns of variable volume from 1 to 20 ml as required Each HiTrap™ column is equiped with one 1/16" male luer and one 1/16" female luer.HiTrap Capto adhere is a multimodal anion exchanger.
HiTrap columns are available in 1 ml and 5 ml bed volume Q (anion exchange), S or SP (cation exchange) are fully charged over a broad pH range.
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